Cyclin d1 t286a
WebApr 21, 2000 · Human cyclin D1-T286A-T288A mutant was generated in the cyclin D1-pCDNA3 plasmid by site-directed mutagenesis as described previously ( 13. ). Transfections were performed using Fugene6 TM following the manufacturer's protocol (Roche Molecular Biochemicals). In Vitro Ubiquitination Assay WebNov 15, 2016 · We found Cyclin D1 undergoes ubiquitinated degradation in berberine-treated HepG2 cells, and phosphorylation at Thr-286 site of Cyclin D1 is required for berberine-driven Cyclin D1 degradation. The β-transducin repeat-containing protein (β-TrCP) recruitment as E3 ligases by berberine are induced when Cyclin D1 proteolyzes.
Cyclin d1 t286a
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WebJun 6, 2014 · f-g) Cyclin D1 T286A overexpression reduces gluconeogenic genes and glycemia in high fat diet fed mice (two-tailed unpaired t-test, n=6/GFP, n=7/D1 T286A). h) Cyclin D1 and Rb phosphorylation levels in livers of high fat diet fed mice that were tail-vein injected with adenoviruses expressing GFP or cyclin D1 T286A, shown in Extended … WebRat-1 cells, cyclin D1 mutant (T286A) that is resistant to ubiq-uitin-proteasome degradation was transfected into the Rat-1: iRas cells. Expression of cyclin D1(T286A) resulted in a sus-tained transformed phenotype with no accumulation of cells in G 1. Inhibition of MAP kinase pathway partially inhibited the Ras-mediated down-regulation of ...
WebApr 21, 2000 · Human cyclin D1-T286A-T288A mutant was generated in the cyclin D1-pCDNA3 plasmid by site-directed mutagenesis as described previously ( 13. ). … http://yuxiqbs.cqvip.com/Qikan/Search/Index?key=S%3d%e5%8c%97%e4%ba%ac%e5%a4%a7%e5%ad%a6%e5%9f%ba%e7%a1%80%e5%8c%bb%e5%ad%a6%e9%99%a2%e7%97%85%e5%8e%9f%e7%94%9f%e7%89%a9%e5%ad%a6%e7%b3%bb%e6%9a%a8%e6%84%9f%e6%9f%93%e7%97%85%e7%a0%94%e7%a9%b6%e4%b8%ad%e5%bf%83
WebThe percentage of cells expressing exclusively cytoplasmic cyclin D1 or D1-(T286A) is presented graphically and represents the average of at least four independent experiments. Vertical bars indicate standard deviations from the mean. On the basis of these results, we considered the possibility that phosphorylation of cyclin D1 at Thr-286 might ... WebJul 6, 2007 · NIH3T3 Flag D1 T286A cells, graciously provided by Dr. Alan Diehl, University of Pennsylvania, were maintained in DMEM supplemented with 10% FBS, glutamine, antibiotics, and puromycin (7.5 μg/mL; BD …
WebImmunoprecipitation analysis revealed that cyclin D1a and cyclin D1-T286A could bind to cyclin-dependent kinase 4 (CDK4) but cyclin D1b has lost its capacity to associate with …
Webthe EcoRI fragment of D1 T286A does not contain 3'UTR" - insert is ~ 0.3 kb smaller than the wild type mouse cyclinD1- the FLAG-tagged cDNA can be recovered with BamH1 digestion. How to cite this plasmid ( Back to top ) … nuby happy hands teething mittenWebDec 14, 2024 · All Names: BCL-1 oncogene, BCL1, CCND1, CYL-1, G1/S-specific cyclin D1, PRAD1, PRAD1 oncogene Form Of Antibody: Rabbit IgG in phosphate buffered … nimishillen township fire deptWebAug 8, 2024 · To detect whether cyclin D1 attributed to the NTCP down-regulation in proliferating cells, we transfected the constitutively active T286A mutant of cyclin D1 into … nuby handy hookWebAll lanes : Anti-Cyclin D1 (phospho T286) antibody (ab62151) at 1/500 dilution Lane 1 : Extracts from Jurkat cells treated with EGF (200ng/ml, 30mins) Lane 2 : Extracts from … nuby hair serumWebJul 20, 1999 · NIH 3T3 cells were maintained in DMEM supplemented with 10% (vol/vol) FCS, antibiotics, and glutamine (GIBCO). NIH 3T3 cells engineered to overexpress Flag … nuby hairstylingWebAutophagy-selective degradation of ubiquitinated cyclin D1 through SQSTM1 was confirmed using cyclin D1/ubiquitin binding site (K 33-238 R) and phosphorylation site (T286A) mutants, lentivirus-mediated silencing autophagy-related 5 (ATG5), autophagy-related 7 (ATG7), and Sqstm1 knockout cells. Functional studies revealed that autophagy ... nimishillen township ohio zoningWebCyclin D1 is a 36-kDa protein located on chromosome 11q13, a region commonly amplified in breast cancer ( Fantl, Smith, Brookes, Dickson, & Peters, 1993). Cyclin D1 functions in regulation of progression of the cell cycle through the G1 to S phase and in cellular metabolism (Fu, Wang, Li, Sakamaki, & Pestell, 2004 ). nimishillen township ohio website