WebFeb 26, 2024 · The Halberd sequence is quite distinct from that of Spica with many amino acid changes, several of which were predicted to affect the protein structure. These include V324E, the Valine at this position is >90% conserved among the 101 members of the ent-copalyl diphosphate synthase gene family, D331G (D, 100% conserved) and V493A … WebApr 8, 2024 · Meanwhile, the diterpenoid-related genes in tea plants have recently been reported, including one CPS (ent-copalyl diphosphate synthase, CPS) and two highly similar KSs (ent-kaurene synthase, KS) . In fast-growing tissues, such as tender stems and roots, the relative expression levels of CsCPS (MN961684) and CsKSs (MN961685; …
Structure and mechanism of the diterpene cyclase ent …
WebRecombinant expression and functional analysis demonstrated that both AN1 and AN2 are ent -copalyl diphosphate ( ent -CPP) synthases ( ent -CPS). Notably, the presence of … Webent-Kaurene is synthesized by the two-step cyclization of geranylgeranyl diphosphate (GGDP) via the intermediate, copalyl diphosphate (CDP). The enzymes that catalyze these reactions are referred to as the A and B activities, respectively, of ent … ehob positioning wedges
Cloning and Characterization of cDNAs Encoding ent-Copalyl …
WebTo assess how up-regulation of early steps affects the biosynthetic pathway overall, we have examined transgenic Arabidopsis plants that overexpress either AtCPS or AtKS or both. … WebJan 11, 2016 · Substitution of a histidine, comprising part of the catalytic base group in the ent-copalyl diphosphate synthases found in all seed plants for gibberellin phytohormone metabolism, by a larger aromatic residue leads to rearrangements. Through a series of 1,2-hydride and methyl shifts of the initially … WebIn enzymology, an ent-copalyl diphosphate synthase (EC 5.5.1.13) is an enzyme that catalyzes the chemical reaction: Hence, this enzyme has one substrate, … ehob pre-inflated cushion