WebTissue was then mechanically dissociated with the gentleMACS TM Dissociator (MACS Milteny Biotec) using C-tubes for 2 minutes. Subsequently, the sample was filtered through a 70 μm strainer (BD Falcon) and spun down for 5 min at 300 g at 4°C. Cells were re-filtered through a 40- μm strainer, spun down for 5 min at 300 g at 4°C and re ... WebMechanical dissociation was accomplished by performing three consecutive automated steps on the gentleMACS TM Dissociator (h_tumor_01, h_tumor_02, and h_tumor_03).
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WebGentle MACS Dissociator. The gentleMACS™ Octo Dissociator with Heaters is a benchtop instrument for the fully automated and standardized tissue dissociation or … WebgentleMACS™ M Tubes are used in combination with the gentleMACS Dissociators for the fast and convenient automated tissue homogenization, in order to isolate biomolecules … black martha\u0027s vineyard rentals
gentleMACS™ Tissue Dissociators and Tubes
WebJun 18, 2024 · Attach the gentleMACS C Tube upside down onto the sleeve of the gentleMACS Dissociator. 13. Run the gentleMACS program h_tumor_02. 14. After termination of the program, detach the gentleMACS C Tube from the gentleMACS Dissociator. 15. Incubate each sample for 30 min at 37°C under continuous rotation … WebgentleMACS Dissociator (130-093-235) Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation using the gentleMACS™ … Webhomogenized into single cell suspensions using the gentleMACSTM Octo Dissociator (Miltenyi Biotech). Cells were incubated with a cocktail of anti-human antibody conjugates for surface marker staining. For intracellular staining, cells were fixed and permeabilized using the Foxp3 staining buffer set (ThermoFisher). Prior to intracellular ... blackmart review