WebSchéma explicatif de l'opéron lactose. L' opéron lactose, ou opéron lac est un opéron nécessaire au transport et au métabolisme du lactose chez Escherichia coli, ainsi que d'autres bactéries de la flore intestinale. L'opéron lactose est composé de trois gènes structurels : lacZ, lacY et lacA. Il est régulé par plusieurs facteurs ... WebSodium citrate (FW = 43.7 g/mol) SDS powder (FW = 288.38 g/mol) 0.5M EDTA IPTG powder (FW = 238.3 g/mol) 20mg/mL X-gal solution in DMF 1000X ONPG Premade LB liquid broth Premade LB agar 0.5mM Colchicine 50X gel electrophoresis running buffer Agarose powder 95% ethanol 100mg/mL Spectinomycin 10X BamHI restriction enzyme digestion buffer
Iptg X Gal at Thomas Scientific
WebApr 3, 2024 · IPTG 常用于需要 诱导 beta-半乳糖苷酶活性的克隆实验。 它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。 IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码 基因 … WebPreparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening For individual LB (Luria Broth) agar plates: 1. Pour sterile warm LB agar (about 25 mL) into a Petri dish. 2. … cytology ascus
X-Gal and IPTG - Takara Bio
WebPlate labels Plate Additives Showed Growth Color of Colonies A IPTG, X-gal, spectinomycinYes Blue B IPTG, X-gal, spectinomycinYes Blue C IPTG, X-gal, spectinomycinYes Blue D IPTG, X-gal, spectinomycinYes Blue 8 All the bacterial plate cultures had colonies, indicating that growth had occurred. (Sehgal et al, 2024). WebOur X-Gal and IPTG products are intended to be used together for blue-white colony screening. X-Gal (5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactoside) is a chromogenic … WebJun 4, 2015 · (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x … bing chat gpt summarize annual report